Composite

Part:BBa_K2638205:Design

Designed by: Jakob Zubek   Group: iGEM18_Bielefeld-CeBiTec   (2018-09-02)


OprC (TonB dependent copper transport porin, BBa_K2638200) with CueR and copAP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 562
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 562
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 562
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 562
    Illegal NgoMIV site found at 824
    Illegal NgoMIV site found at 1155
    Illegal NgoMIV site found at 1359
    Illegal NgoMIV site found at 2403
    Illegal NgoMIV site found at 2604
    Illegal AgeI site found at 818
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 475


Design Notes

This biobrick is a positive feedback loop, which causes the cell to take up a lethal dose of copper.


Source

Escherichia Coli K12 and Pseudomonas brassicacearum 3Re2-7 13

References